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dna sequences for enst00000198536.7 (wildtype) and enst00000198536.7 containing rs2405442:t>c (mutant)  (GenScript corporation)

 
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    Structured Review

    GenScript corporation dna sequences for enst00000198536.7 (wildtype) and enst00000198536.7 containing rs2405442:t>c (mutant)
    Dna Sequences For Enst00000198536.7 (Wildtype) And Enst00000198536.7 Containing Rs2405442:T>C (Mutant), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+dna+sequence/dna+sequences+for+enst00000198536+7++wildtype++and+enst00000198536+7+containing+rs2405442+t+c++mutant+/pm40149715-59-6-13
    Average 90 stars, based on 1 article reviews
    dna sequences for enst00000198536.7 (wildtype) and enst00000198536.7 containing rs2405442:t>c (mutant) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Hotspots of missense mutation identify novel neurodevelopmental disorder genes and functional domains
    Article Snippet: Patient samples were acquired from Adelaide (Jozef Gecz, University of Adelaide), Antwerp (Frank Kooy, University of Antwerp), Autism Clinical and Genetic Resources in China (ACGC; Kun Xia), the Autism Genetic Resource Exchange (AGRE), Iowa (Jacob Michaelson, University of Iowa), Leiden (Gijs Santen, Leiden University Medical Center), Leuven (Hilde Peeters, University Hospitals Leuven), Philadelphia (Hakon Hakonarson, Children’s Hospital of Philadelphia), Prague (Zdenek Sedlacek, University Hospital Motol), San Diego (Eric Courchesne, UC San Diego), Simons Simplex Collection (SSC), Stockholm (Magnus Nordenskjold, Karolinska University Hospital), the Study of Autism Genetics Exploration (SAGE; Raphe Bernier, University of Washington), The Autism Simplex Collection (TASC), and Troina (Corrado Romano, Associazione Oasi Maria Santissima). .. GRIA1 wild-type and A636T mutant DNA sequences were synthesized (GenScript) and cloned into mammalian expression vectors. .. Human embryonic kidney (HEK) 293T/17 SF (ATCC ACS-4500) cells, routinely used for transient transfection and electrophysiological recordings as they allow robust heterologous expression, were cultured in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum and 1% Streptomycin up to a maximum passage number of 15.

    Article Title: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
    Article Snippet: .. The LV_AkaLuc vector used for brain orthotopic transplantation was constructed using synthesized firefly luciferase mutant protein AkaLuc (GenBank, LC320664.1 ) purchased from Genscript. ..

    Article Title: Human Saposin B Ligand Binding and Presentation to α-Galactosidase A
    Article Snippet: Before all assays, the saposin protein was spun at 16,000 g for 10 min and buffer exchanged over a Zeba spin desalting column (ThermoFisher) with a 7 kDa MWCO into the appropriate buffer. .. Sf9 expressed GLA: We purchased from Genscript the codon-optimized wild-type and D170A mutant human GLA (UniProt P06280) nucleotide sequences inserted between the Csp I and Hin dIII sites of the expression vector pFastBac1. ..

    Synthesized:

    Article Title: Hotspots of missense mutation identify novel neurodevelopmental disorder genes and functional domains
    Article Snippet: Patient samples were acquired from Adelaide (Jozef Gecz, University of Adelaide), Antwerp (Frank Kooy, University of Antwerp), Autism Clinical and Genetic Resources in China (ACGC; Kun Xia), the Autism Genetic Resource Exchange (AGRE), Iowa (Jacob Michaelson, University of Iowa), Leiden (Gijs Santen, Leiden University Medical Center), Leuven (Hilde Peeters, University Hospitals Leuven), Philadelphia (Hakon Hakonarson, Children’s Hospital of Philadelphia), Prague (Zdenek Sedlacek, University Hospital Motol), San Diego (Eric Courchesne, UC San Diego), Simons Simplex Collection (SSC), Stockholm (Magnus Nordenskjold, Karolinska University Hospital), the Study of Autism Genetics Exploration (SAGE; Raphe Bernier, University of Washington), The Autism Simplex Collection (TASC), and Troina (Corrado Romano, Associazione Oasi Maria Santissima). .. GRIA1 wild-type and A636T mutant DNA sequences were synthesized (GenScript) and cloned into mammalian expression vectors. .. Human embryonic kidney (HEK) 293T/17 SF (ATCC ACS-4500) cells, routinely used for transient transfection and electrophysiological recordings as they allow robust heterologous expression, were cultured in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum and 1% Streptomycin up to a maximum passage number of 15.

    Article Title: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
    Article Snippet: .. The LV_AkaLuc vector used for brain orthotopic transplantation was constructed using synthesized firefly luciferase mutant protein AkaLuc (GenBank, LC320664.1 ) purchased from Genscript. ..

    Clone Assay:

    Article Title: Hotspots of missense mutation identify novel neurodevelopmental disorder genes and functional domains
    Article Snippet: Patient samples were acquired from Adelaide (Jozef Gecz, University of Adelaide), Antwerp (Frank Kooy, University of Antwerp), Autism Clinical and Genetic Resources in China (ACGC; Kun Xia), the Autism Genetic Resource Exchange (AGRE), Iowa (Jacob Michaelson, University of Iowa), Leiden (Gijs Santen, Leiden University Medical Center), Leuven (Hilde Peeters, University Hospitals Leuven), Philadelphia (Hakon Hakonarson, Children’s Hospital of Philadelphia), Prague (Zdenek Sedlacek, University Hospital Motol), San Diego (Eric Courchesne, UC San Diego), Simons Simplex Collection (SSC), Stockholm (Magnus Nordenskjold, Karolinska University Hospital), the Study of Autism Genetics Exploration (SAGE; Raphe Bernier, University of Washington), The Autism Simplex Collection (TASC), and Troina (Corrado Romano, Associazione Oasi Maria Santissima). .. GRIA1 wild-type and A636T mutant DNA sequences were synthesized (GenScript) and cloned into mammalian expression vectors. .. Human embryonic kidney (HEK) 293T/17 SF (ATCC ACS-4500) cells, routinely used for transient transfection and electrophysiological recordings as they allow robust heterologous expression, were cultured in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum and 1% Streptomycin up to a maximum passage number of 15.

    Expressing:

    Article Title: Hotspots of missense mutation identify novel neurodevelopmental disorder genes and functional domains
    Article Snippet: Patient samples were acquired from Adelaide (Jozef Gecz, University of Adelaide), Antwerp (Frank Kooy, University of Antwerp), Autism Clinical and Genetic Resources in China (ACGC; Kun Xia), the Autism Genetic Resource Exchange (AGRE), Iowa (Jacob Michaelson, University of Iowa), Leiden (Gijs Santen, Leiden University Medical Center), Leuven (Hilde Peeters, University Hospitals Leuven), Philadelphia (Hakon Hakonarson, Children’s Hospital of Philadelphia), Prague (Zdenek Sedlacek, University Hospital Motol), San Diego (Eric Courchesne, UC San Diego), Simons Simplex Collection (SSC), Stockholm (Magnus Nordenskjold, Karolinska University Hospital), the Study of Autism Genetics Exploration (SAGE; Raphe Bernier, University of Washington), The Autism Simplex Collection (TASC), and Troina (Corrado Romano, Associazione Oasi Maria Santissima). .. GRIA1 wild-type and A636T mutant DNA sequences were synthesized (GenScript) and cloned into mammalian expression vectors. .. Human embryonic kidney (HEK) 293T/17 SF (ATCC ACS-4500) cells, routinely used for transient transfection and electrophysiological recordings as they allow robust heterologous expression, were cultured in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum and 1% Streptomycin up to a maximum passage number of 15.

    Article Title: Human Saposin B Ligand Binding and Presentation to α-Galactosidase A
    Article Snippet: Before all assays, the saposin protein was spun at 16,000 g for 10 min and buffer exchanged over a Zeba spin desalting column (ThermoFisher) with a 7 kDa MWCO into the appropriate buffer. .. Sf9 expressed GLA: We purchased from Genscript the codon-optimized wild-type and D170A mutant human GLA (UniProt P06280) nucleotide sequences inserted between the Csp I and Hin dIII sites of the expression vector pFastBac1. ..

    Plasmid Preparation:

    Article Title: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
    Article Snippet: .. The LV_AkaLuc vector used for brain orthotopic transplantation was constructed using synthesized firefly luciferase mutant protein AkaLuc (GenBank, LC320664.1 ) purchased from Genscript. ..

    Article Title: Human Saposin B Ligand Binding and Presentation to α-Galactosidase A
    Article Snippet: Before all assays, the saposin protein was spun at 16,000 g for 10 min and buffer exchanged over a Zeba spin desalting column (ThermoFisher) with a 7 kDa MWCO into the appropriate buffer. .. Sf9 expressed GLA: We purchased from Genscript the codon-optimized wild-type and D170A mutant human GLA (UniProt P06280) nucleotide sequences inserted between the Csp I and Hin dIII sites of the expression vector pFastBac1. ..

    Transplantation Assay:

    Article Title: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
    Article Snippet: .. The LV_AkaLuc vector used for brain orthotopic transplantation was constructed using synthesized firefly luciferase mutant protein AkaLuc (GenBank, LC320664.1 ) purchased from Genscript. ..

    Construct:

    Article Title: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
    Article Snippet: .. The LV_AkaLuc vector used for brain orthotopic transplantation was constructed using synthesized firefly luciferase mutant protein AkaLuc (GenBank, LC320664.1 ) purchased from Genscript. ..

    Luciferase:

    Article Title: BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network
    Article Snippet: .. The LV_AkaLuc vector used for brain orthotopic transplantation was constructed using synthesized firefly luciferase mutant protein AkaLuc (GenBank, LC320664.1 ) purchased from Genscript. ..

    other:

    Article Title: Oxidation-sensitive cysteines drive IL-38 amyloid formation.
    Article Snippet: The full-length WT or mutant human IL-38 cDNA sequence was codon optimized for E. coli, synthetized and cloned by GenScript Biotech into a pET21a vector (Addgene, Watertown, MA, USA) in order to add a TEV protease cleavable N-terminal 6xHis tag.

    Article Title: The Hydrogen-Coupled Oligopeptide Membrane Cotransporter Pept2 is SUMOylated in Kidney Distal Convoluted Tubule Cells
    Article Snippet: Stably expressing cells were selected using 2 μg/ml puromycin (Sigma-Aldrich) and named mpkDCT-SUMO1 T95K or mpkDCT-SUMO2 T90K cells. mpkDCT cells were also transfected with wild-type or K139R-mutant FLAG-tagged Pept2 (Genscript, DNA sequences in ) using Lipofectamine 2000 (Invitrogen).



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    Image Search Results


    Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

    Journal: Developmental cell

    Article Title: Pancreatic alpha and beta cell fate choice is directed by apical-basal polarity dynamics.

    doi: 10.1016/j.devcel.2025.02.008

    Figure Lengend Snippet: Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Latrunculin B Sigma Aldrich #L5288 Heparin Sigma Aldrich #H3149-100KU Bovine serum albumin (BSA) Proliant #7500804 Bovine serum albumin (BSA) Roche #10775835001 Bovine serum albumin (BSA) Sigma Aldrich #B4287 Y-27632 Merck Millipore #688000 45 % glucose solution in water Sigma Aldrich #G8769 7.5% sodium bicarbonate solution Thermo Fisher #25080094 Forskolin Sigma Aldrich #F6886 3-Isobutyl-1-methylxanthin Thermo Fisher #J64598.MC G418 Thermo Fisher #10131027 Hygromycin Thermo Fisher #10687010 Doxycycline Sigma-Aldrich #D3447 Critical commercial assays RNeasy Mini Kit Qiagen #74106 RNeasy Micro Kit Qiagen #74004 iScript cDNA Synthesis Kit BIO-RAD #1708891 KAPA HiFi HotStart Library Amplification Kit Roche #07958951001 cAMP XP@ Assay Kit Cell Signaling #4339 P3 Primary Cell Kit Lonza #V4XP-3024 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher #L34964 Deposited data scRNA-seq datasets This paper GSE224286 Experimental models: Cell lines SA121 Takara bio N/A H1 Beydag-Tasöz et al.34 (University of Copenhagen) N/A Experimental models: Organisms/strains Cdc42fl/fl R26-YFP+/+ mice In house N/A Pdx1-Cre+/- Cdc42fl/wt mice In house N/A Oligonucleotides siRNA against EGR1 Thermo Fisher #S4537; #S4538 Negative control siRNA Thermo Fisher #4390843 Recombinant DNA Mutant CDH1 sequences Addgene #45770; #45773 PiggyBac destination vector PB-TAC-ERN Addgene #80475 Cas9 expression vector (pX458-HF1) Addgene #48138 Software and algorithms Adobe Photoshop 2022 Adobe https://www.adobe.com Adobe Illustrator 2023 Adobe https://www.adobe.com Imaris 8.4, 9.0 Oxford Instruments https://imaris.oxinst.com/ Fiji 2.0/ImageJ NIH Image http://imagej.nih.gov/ij GraphPad Prism 10 GraphPad https://www.graphpad.com FlowJo 10 BD https://www.flowjo.com FCS Express 7 De Novo Software https://denovosoftware.com ZEN (blue edition) ZEISS https://www.micro-shop.zeiss.com Developmental Cell 60, 1–13.e1–e5, July 7, 2025 e2

    Techniques: Disruption, Dominant Negative Mutation, Knock-Out, Binding Assay, Flow Cytometry, Mutagenesis